Occurrence of glutamate synthase in Brevibacterium flavum.
نویسندگان
چکیده
منابع مشابه
Threonine production by dihydrodipicolinate synthase-defective mutants of Brevibacterium flavum.
A novel type of threonine-producing strains, dihydrodipicolinate synthase (DPS)-defective mutants of Brevibacterium flavum, was isolated as alpha-amino-beta-hydroxyvaleric acid (AHV)-resistant producers. The third selection markers used were a strong lysine inhibition of threonine production and a lower production of lysine than that of threonine in those derived from strains with feedback-sens...
متن کاملOptimization of Physico-chemical Parameters for Hyper- Production of Lysine by Mutated Strain of Brevibacterium Flavum
Lysine as an essential amino acid supplemented to fulfil the dietary requirement of Poultry feed. In Pakistan all this demand is accomplished by importing lysine for feed sector. Wild Brevibacterium flavum was used to induce mutation by random mutagenesis for hyper production of lysine through fermentation. The highest lysine producing strain (BFENU2) was screened with 21.64 g/L production by m...
متن کاملTissue Distribution of Glutamate Synthase and Glutamine Synthetase in Rice Leaves : Occurrence of NADH-Dependent Glutamate Synthase Protein and Activity in the Unexpanded, Nongreen Leaf Blades.
To further explore the function of NADH-dependent glutamate synthase (GOGAT), the tissue distribution of NADH-GOGAT protein and activity was investigated in rice (Oryza sativa L.) leaves. The distributions of ferredoxin (Fd)-dependent GOGAT, plastidic glutamine synthetase, and cytosolic glutamine synthetase proteins were also determined in the same tissues. High levels of NADH-GOGAT protein (33...
متن کاملAnalysis of Functional Domains on Glutamate Synthase
Glutamate synthases (GOGAT) were analyzed to identify the functional binding domains of the substrate (glutamine) and cofactors (FMN, NAD(P)H, FAD, [3Fe-4S] and [4Fe-4S] clusters and ferredoxin) on this enzyme. The published amino acid sequences of six different NAD(P)Hdependent GOGATs (NAD(P)H-GOGAT) and ten different ferredoxin-dependent GOGATs (Fd-GOGAT) were used for this analysis. The amin...
متن کاملA polarographic study of glutamate synthase activity in isolated chloroplasts.
Illuminated pea (Pisum sativum) chloroplasts actively catalyzed (glutamine plus alpha-ketoglutarate)-dependent O(2) evolution (average of 12 preparations 10.6 mumole mg chlorophyll per hour). The reaction was specific for glutamine and alpha-ketoglutarate; concentrations of 0.2 mm alpha-ketoglutarate and 0.6 mm glutamine, respectively, effected half-maximum rates of O(2) evolution. The reaction...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1984
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.48.2149